Trypsin S-SAD Phasing: How Low (In Frames) Can You Go?

This dataset was recorded from a trypsin crystal for S-SAD phasing (calcium is also present). 3600 out of a total of 7200 frames were uploaded. Phasing is straightforward with 3600 frames included, however 1800 is more difficult to build a complete model.

Useful Information

Data kindly provided by: Prof. Min Yao at Hokkaido University, Japan.
Sample: Trypsin
Beamline where data were collected: BL-1A, Photon Factory
Wavelength: 2.7 Å
Detector: Pilatus 2M
Oscillation angle: 0.1 degree
Exposure: 0.1 sec/frame
Distance: 65 mm
Beam size: 10 x 25 µm²
Image file format: .cbf file extension (crystalographic binary file)
Protein sequence in FASTA format

Two options to download the data:

Option 1 (easiest):

Use 'rsync' (linux/UNIX command) to download the compressed image files (command is in between quotes and should only be one line). There are 3600, but each file is approximately 270 kb:

"rsync -azv anonymous@bl831.als.lbl.gov:pub/ACA2014/Min.Yao_trypsin_4.6keV/ ."

and you will be prompted to enter 'lysozyme'.

Option 2:

Use Wget to download the 'wloop_1_#####.cbf.bz2' images (try this if you have a Windows OS).

Processed Data and Instructions

	HKL-3000	iMosflm	XDS	PROTEUM2
Processed Data Files	TBA	TBA	TBA	TBA
Log Files	TBA	TBA	TBA	TBA
Author Instructions	Wladek Minor	Harry Powell	Keitaro Yamashita	Michael Ruf
SFCheck Output	For HKL-3000 file	For iMosflm file	For XDS file	For PROTEUM2 file