Tough Spots
This dataset was recorded from platelike crystals that were typically thin in the third dimension. Data from these crystals diffracted to better than 2 Å resolution and could be solved by MR with a highly-identical search model, however the Rfactor and Rfree values were stuck above 0.30 despite no obvious errors or omissions in the electron density. Possible problems with anisotropic diffraction, high mosaicity and alignment of the long unit cell axis with the rotation axis contributing to anisotropic data quality that potentially manifests in high residuals during refinment.
Useful Information
A limited number of images are available for inspection below in order to demonstrate the nature of the difficult diffraction. Image file format: bzipped .img files
Two options to download the data:
- Option 1 (easiest):
Use 'rsync' (linux/UNIX command) to download the compressed image files (command is in between quotes and should only be one line). There are only 10 frames available for now in order to view the nature of the undesirable spot profiles:
and you will be prompted to enter 'lysozyme'.
- Option 2:
Use Wget to download the '' images (try this if you have a Windows OS).
