Difficult High-resolution Triclinic Lysozyme
Very high resolution data can present a challenge for processing, as exemplified by this dataset from a crystal of triclinic hen egg-white lysozyme collected at 0.65 angstroms resolution. NOTE: This dataset is very large (>120 Gb), so only a small fraction (4.2 Gb) will be used for the demonstration (filename root: 'p1lyso_a.####.img'). Be sure to type in the 'rsync' commands accurately below to avoid downloading the whole dataset.
Useful Information
Data kindly provided by: Zbigniew Dauter
Sample: Hen egg-white lysozyme (triclinic)
PDB Code: 2VB1
Date data were collected: June 30, 2006
Beamline where data were collected: APS beamline 19ID
Image file format: The image files were collected on an ADSC Quantum 315 detector.
Four options to download the data:
- Option 1 (easiest):
Use 'rsync' (linux/UNIX command) to download only the first 3 (of 63!) tarred/compressed image bundles (command is in between quotes and should only be one line):
and you will be prompted to enter 'lysozyme'.
- Option 2:
Use 'rsync' (linux/UNIX command) to download only the first 60 (of >1500!) images (command is in between quotes and should only be one line):
and you will be prompted to enter 'lysozyme'.
- Option 3:
Use Wget to download only the 'p1lyso_a.####.img' images (try this if you have a Windows OS).
- Option 4:
Download either the compressed image file bundles (preferred) or the individual image files (a cumbersome last resort; you probably want the compressed file bundles instead, but if you're absolutely sure, remember to only download the 'p1lyso_a.####.img files!) using your web browser.
Processed Data and Instructions
| CrystalClear | Elves | HKL-2000 | iMosflm | XDS | autoPROC | PROTEUM2 | |
|---|---|---|---|---|---|---|---|
| Processed Data Files | TBA | 2vb1_all.mtz | TBA | TBA | XDS results | autoPROC results | TBA |
| Log Files | TBA | TBA | TBA | TBA | Links on this page | Excerpt at end of this page | TBA |
| Author Instructions | Jim Pflugrath | James Holton | Wladek Minor | Harry Powell | Kay Diederichs | Clemens Vonrhein | Matt Benning |
| SFCheck Output | For CrystalClear file | For Elves file | For HKL-2000 file | For iMosflm file | XDS SFCHECK file | For autoPROC file | For PROTEUM2 file |
