Challenging Spots example
We often collect data that, for a variety of reasons, contain spots with less-than-ideal shape/properties. This dataset is a good example of a 'typical' membrane protein dataset with a long unit cell axis. Note that the data for this example are organized in three directories representing the three wavelengths used for collection: peak wavlength ('pk'), inflection point ('ip') and high-remote wavelength ('hrem'). All three wavelengths will be necessary for successful phasing.
Useful Information
Data kindly provided by: Kay Diederichs, see details of the data here
Sample: Hemophore receptor HasR from Serratia marcescens in complex with its hemophore HasA and heme.
PDB Code: 3CSL
Dates data were collected: April 29, 2006 and November 26, 2006
Beamlines where data were collected: ESRF ID29 and SLS X06SA
Image file formats: The image files were collected on a ADSC Quantum 315 or a MarMosaic 225 detector.
Four options to download:
- Option 1 (easiest):
Use 'rsync' (linux/UNIX command) to download all the tarred/compressed image bundles at once (command is in between quotes):
and you will be prompted to enter 'lysozyme'.
- Option 2:
Use 'rsync' (linux/UNIX command) to download all the image files at once (command is in between quotes):
and you will be prompted to enter 'lysozyme'.
- Option 3:
Use Wget to download the images (try this if you have a Windows OS).
- Option 4:
Download either the compressed image file bundles (preferred) or the individual image files (a cumbersome last resort; you probably want the compressed file bundles instead) using your web browser.
Processed Data and Instructions
| CrystalClear | Elves | HKL-2000 | iMosflm | XDS | autoPROC | PROTEUM2 | |
|---|---|---|---|---|---|---|---|
| Processed Data Files | TBA | TBA | TBA | TBA | XDS results | autoPROC results | TBA |
| Log Files | TBA | TBA | TBA | TBA | Links on this page | Excerpt on this page | TBA |
| Author Instructions | Jim Pflugrath | James Holton | Wladek Minor | Harry Powell | Kay Diederichs | Clemens Vonrhein | Matt Benning |
| SFCheck Output | For CrystalClear file | For Elves file | For HKL-2000 file | For iMosflm file | For XDS file | For autoPROC file | For PROTEUM2 file |
