A Few Useful SCRIPTS are here.
Data Processing notes are here.
Data Collection Notes
Holton's recommendations for data collection, taken from his Letter Introducing the New Pilatus Detector
Holton's Crystal Size Calculator
Holton's Crystal Lifetime Calculator
run "cellvolume.com" from the command line to determine if you have a detectable signal for flurescence scans (>~10 mMolar is required)
Some scripts which may be of use
(the links are only to view the scripts themselves, copy and paste the script "name" to run it:
Calculate the best exposure time based on the current, logged, or any other Radiation Dose Rate.
You'll need to know the maximum dose you can tolerate (#Mega or #Kilo Grays), the expected dose rate (Grays/sec), and the number of frames.
An exposure time per frame will be calculated.
Defaults will be suggested based on last on the flux of the last tuneup and any run information that is in the Run_1 BLU-ICE window.
The script is interactive and defaults will be suggested.
1) You will be asked it you want to measure and use the current Dose Rate (flux) NOW,
- OR- the flux at the last tune up,
- OR - you can enter any other value to use (in Grays per second).
2) You'll be asked for the maximum Dose you want.
Expected lifedose of typical protein crystals:
---> Native 30000000 (7 zeros)
---> MAD 5000000 (6 zeros)
---> S SAD 3000000 (6 zeros)
---> Br/RNA 1000000 (6 zeros)
---> RT 200000 (5 zeros)
3) finally you'll be asked how many frames will be in the run.
A Quasi-Vector Scan: A script to setup data collection at a series of different points along a crystal.
. . . run "waypoint_setup.com" from the command line
Calculate the best exposure time based on current or calculated Radiation Dose Rate for different types of measurements.
run "exposure_time.com" from the command line
Calculate the slit postition to achive a desired Attenuation of the Beam to reduce the Radiation Dose Rate
run "calculate_atten.com" from the command line
Heavy Atom Collection Notes
Recommedations for collecing MAD data, in particular Mercury
Collecting Sulfur SAD data
I. Collecting Bromine data
II. Collecting Bromine data
Where to collect? At the K Edge or L-III Edge?
How dangerous are Mecury Soaked Protein Samples (Not very!) Holton's Anchovie Analysis
Collecting Iodinated RNA data
Collecting at the Strontium Edge
Collecting Lanthonide data
A Beginner's Guide to Radiation Damage
Radiation Damage Rates: James Holton's, "How long will my crystal last?"