8/3/2016 User asks: Elves do not handle Pilatus data well. Perhaps due to MOSFLM, as you mentioned. Do you have XDS_Elves to process data? Would like to download it to my lab computer. James replies: I'm afraid I haven't written that! This is mostly because the field of "neat front-ends to XDS" is already rather saturated. Have you tried "xia2"? Graeme Winter took many of my philosophies of Elves in developing it, and it is now part of CCP4. You just run "xia2 -3d /data/whatever/" and it should figure out the image patterns, run XDS, and merge it all together with aimless. Let me (and Graeme) know if you have any trouble with that? -James ********************************************************* 8/3/2016 User: Thank you, James. Will try that. Let me also ask about the status of the truncated.mtz file generated at the beamline by xds_runme.com. Is any further processing of the truncated.mtz required? It seems already merged James replied: Yes, truncated.mtz is the scaled, merged and truncated data. Similar to the "merged.mtz" output by Elves, except that it is using XDS/XSCALE/XDSCONV instead of mosflm/scala/truncate. I gave it a different name to make it easier to compare the results. ********************************************************* User: Excellent! It took almost no time to process the dataset at the beamline! I wish I knew how to specify frames to eliminate those with the radiation damage for the final processing. One of my crystals sustains only 800 frames, then data statistics notably declined. Would be nice to have this mentioned in instructions. James: We're working on documentation, but the software is changing faster than we can write about it! I have a jiffy script called xds2mtz.com. You can download the latest version here: http://bl831.als.lbl.gov/~jamesh/scripts/xds2mtz.com You run it like this: xds2mtz.com XDS_ASCII.HKL P212121 1-800 2.3A where XDS_ASCII.HKL is the XDS output file you want to scale and merge. P212121 is the desired space group, 1-800 is the range of images you want to use, 2.3A is the desired resolution cutoff. All of these are optional. *********************************************** ...Note you can merge different wedges of data using xds2mtz.com (always make a new directory and cd into it first) xds2mtz.com /home/mydata/wedge1/XDS_ASCII.HKL /home/mcfuser/mydata/wedge2/XDS_ASCII.HKL /home/mcfuser/mydata/XDS_ASCII.HKL